Chitosan이 치주인대, 두개관 및 치은섬유아세포의 성상에 미치는 영향
Effects of chitosan on the chancteristics of periodontal ligament, calvaria cells and gingival fibroblasts
소속 상세정보
김선희/Sun-Hee Kim
권영혁/이만섭/박준봉/허익/Young-Hyuk Kwon/Man-Sup Lee/Joon-Bong Park/Yeek Herr
KMID : 0363019980280010017
Abstract
결 론
치주질환으로 인해 소실된 치주조직을 재생시키는데 도움을 줄 수 있는 보조약제의 개발
을위해 chitosan(Poly-N-Acetyl Glucosaminoglycan)을 치주인대, 두개관 및 치은섬유아세포
에 적용하여 세포증식율, 단백질 합성능, 염기성 인산분해효소 활성도 및 석회화결절 형성능
을 측정하여 다음과 같은 결론을 얻었다.
1. chitosan은 배양초기에는 치주인대세포와 두개관세포의 증식을 촉진시키다가, 각각 배
양 9일, 12일부터는 오히려 이들 세포의 증식을 억제하는 것으로 나타났다. 치은섬유아세포
의 증식은 전반적으로 억제하였으나 통계학적인 유의성은 없었다.
2. chitosan은 배양 3일에는 치주인대세포와 두개관세포의 단백질 합성능을 증가시키다가,
배양 7일에는 단백질 합성능을 감소시켰다. 치은섬유아세포에 대해서는 유의성 있는 영향을
미치지 않았다.
3. chitosan은 배양 3일, 7일에 치주인대세포와 두개관세포의 염기성 인산분해효소 활성도
를 증가시키는 것으로 나타났으나, 배양 7일에 두개관세포의 염기성 인산분해효소 활성도에
대한 영향은 통계학적인 유의성은 없었다. 치은섬유아세포에 대해서는 유의성 있는 영향을
미치지 않았다.
4. chitosan은 두개관세포의 결절형성을 유의성 있게 증가시켰다(P<0.01).
결론적으로 chitosan은 치주인대세포와 두개관세포의 증식, 단백질 합성능 및 염기성 인산
분해효소 활성도에 영향을 미치며, 골형성을 촉진하는 작용을 하는 것으로 나타났으며, 이러
한 결과로부터 chitosan을 치주조직 재생술식에 임상적으로 응용할 수 있으리라 생각한다.
Chitosan, with a chemical structure similar to hyaluronic acid, has been implicated as
a wound healing agent. The purpose of this research was to evaluate the effects of
chitosan on the characteristics of periodontal ligament cells, calvaria cells and gingival
fibroblasts and to define the effects of chitosan on bone formation in vitro.
In control group, the cells were cultured alone with Dulbecco's Modified Eagle's
Medium contained with 10% Fetal bovine serum, 100unit/㎖ penicillin, 100㎍/㎖
streptomycin, 0.5㎍/㎖ amphotericin-B. In experimental group, chitosan(40㎍/㎖) is added
into the abode culture condition. And then each group was characterized by examining
the cell proliferation at 1, 3, 5, 7, 9, 12, 15 day, the amount of total protein synthesis,
alkaline phosphatase activity at 3, 7 day and the ability to produce mineralized nodules
of rat calvaria cell at 11 day.
The results were as follows :
1. At early time both periodontal ligament cells and calvaria cells in chitosan-treated
group proliferated more rapidly than in non-treated control group, but chitosan-treated
group of periodontal ligament cells at 9 days and calvaria cells at 126days showed lower
growth rate than control group.
Gingival fibroblast in chitosan-treated group had lower growth rate than in control
group but the difference was not statistically significant (P< 0.01).
2. Both periodontal ligament cells and calvaria cells in chitosan-treated group showed
much protein synthesis than in control group at 3 days, but showed fewer than in
control group at 7 days. Amount of total protein synthesis of gingival fibroblast didn't
have statistically significant difference among the two groups(P< 0.01).
3. At 3 and 7 days, alkaline phosphatase activity of periodontal ligament cells and
calvria cells was increased in chitosan-treated group, but at 7 days there was not
statistically significant difference among the two groups of calvaria cells (P<0.01).
Alkaline phosphatase activity of gingival fibroblast didn't have statistically significant
difference among the two groups(P<0.01).
4. Mineralized nodules in chitosan-treated group of rat calvaria cells were more than
in control group.
In summery, chitosan had an effect on the proliferation, protein systhesis, alkaline
phosphatase activity of periodontal ligament cell calvaria cell, and facilitated the
formation of bone. It is thought that these effects can be used clinically in periodontal
regeneration therapy.
키워드
원문 및 링크아웃 정보
등재저널 정보